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Article Dans Une Revue BMC Microbiology Année : 2008

Evaluation of an immunomagnetic separation method to capture Candida yeasts cells in blood

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Patricia Licznar
  • Fonction : Auteur
Jane Cottin
  • Fonction : Auteur
Daniel Poulain
  • Fonction : Auteur
Raymond Robert
  • Fonction : Auteur
Corinne Lefrançois
  • Fonction : Auteur
  • PersonId : 992145

Résumé

BackgroundCandida species have become the fourth most-frequent cause of nosocomial bloodstream infections in immunocompromised patients. Therefore, rapid identification of pathogenic fungi to species level has been considered critical for treatment. Conventional diagnostic procedures such as blood culture or biochemical tests are lacking both sensitivity and species specificity, so development of rapid diagnostic is essential. Results An immunomagnetic method involving anti-Candida monoclonal antibodies was developed to capture and concentrate in human blood four different species of Candida cells responsible for invasive yeast infections. In comparison with an automated blood culture, processing time of immunomagnetic separation is shorter, saving at least 24 hours to obtain colonies before identification. Conclusion Thus, this easy to use method provides a promising basis for concentrating all Candida species in blood to improve sensitivity before identification.

Dates et versions

hal-03332251 , version 1 (02-09-2021)

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Citer

Patricia Licznar, Jane Cottin, Daniel Poulain, Raymond Robert, Véronique Apaire-Marchais, et al.. Evaluation of an immunomagnetic separation method to capture Candida yeasts cells in blood. BMC Microbiology, 2008, 8 (1), Non spécifié. ⟨10.1186/1471-2180-8-157⟩. ⟨hal-03332251⟩
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