Caveolin-1-dependent and -independent membrane domains. - Université d'Angers Accéder directement au contenu
Article Dans Une Revue Journal of Lipid Research Année : 2009

Caveolin-1-dependent and -independent membrane domains.

Soazig Le Lay
Qiong Li
  • Fonction : Auteur
Nicholas Proschogo
  • Fonction : Auteur
Macarena Rodriguez
  • Fonction : Auteur
Krishanthi Gunaratnam
  • Fonction : Auteur
Siân Cartland
  • Fonction : Auteur
Carles Rentero
  • Fonction : Auteur
Wendy Jessup
  • Fonction : Auteur
Todd Mitchell
  • Fonction : Auteur
Katharina Gaus
  • Fonction : Auteur


Lipid rafts defined as cholesterol- and sphingomyelin-rich domains have been isolated from different cell types that vary greatly in their lipid profiles. Here, we investigated the contribution of the structural protein caveolin-1 (Cav1) to the overall lipid composition and domain abundance in mouse embryonic fibroblasts (MEFs) from wild-type (WT) or Cav1-deficient (Cav1(-/-)) animals. Our findings show that Cav1 expression had no effect on free (membrane-associated) cholesterol levels. However, Cav1(-/-)-deficient cells did have a higher proportion of sphingomyelin, decreased abundance of unsaturated phospholipids, and a trend toward shorter fatty acid chains in phosphatidylcholine. We isolated detergent-resistant membranes (DRMs), nondetergent raft domains (NDR), and cholesterol oxidase (CO)-sensitive domains and assessed the abundance of ordered domains in intact cells using the fluorescent dye Laurdan. Despite differences in phospholipid composition, we found that cholesterol levels in DRMs, NDR, and CO-sensitive domains were similar in both cell types. The data suggest that Cav1 is not required to target cholesterol to lipid rafts and that CO does not specifically oxidize caveolar cholesterol. In contrast, the abundance of ordered domains in adherent cells is reduced in Cav1(-/-) compared with WT MEFs, suggesting that cell architecture is critical in maintaining Cav1-induced lipid rafts.

Dates et versions

hal-03328705 , version 1 (30-08-2021)



Soazig Le Lay, Qiong Li, Nicholas Proschogo, Macarena Rodriguez, Krishanthi Gunaratnam, et al.. Caveolin-1-dependent and -independent membrane domains.. Journal of Lipid Research, 2009, 50, pp.1609-20. ⟨10.1194/jlr.M800601-JLR200⟩. ⟨hal-03328705⟩


7 Consultations
0 Téléchargements



Gmail Facebook Twitter LinkedIn More