Background and Objective We present NLDyn, an open-source MATLAB toolbox tailored for in-depth analysis of nonlinear dynamics in biomedical signals. Our objective is to offer a user-friendly yet comprehensive platform for researchers to explore the intricacies of time series data. Methods NLDyn integrates approximately 80 distinct methods, encompassing both univariate and multivariate nonlinear dynamics, setting it apart from existing solutions. This toolbox combines state-of-the-art nonlinear dynamical techniques with advanced multivariate entropy methods, providing users with powerful analytical capabilities. NLDyn enables analyses with or without a sliding window, and users can easily access and customize default parameters. Results NLDyn generates results that are both exportable and visually informative, facilitating seamless integration into research and presentations. Its ongoing development ensures it remains at the forefront of nonlinear dynamics analysis. Conclusions NLDyn is a valuable resource for researchers in the biomedical field, offering an intuitive interface and a wide array of nonlinear analysis tools. Its integration of advanced techniques empowers users to gain deeper insights from their data. As we continually refine and expand NLDyn's capabilities, we envision it becoming an indispensable tool for the exploration of complex dynamics in biomedical signals.