<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-03783689</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-13T09:39:19+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">A Descriptive Chemical Composition of Concentrated Bud Macerates through an Optimized SPE-HPLC-UV-MS2 Method—Application to Alnus glutinosa, Ribes nigrum, Rosa canina, Rosmarinus officinalis and Tilia tomentosa</title>
            <author role="aut">
              <persName>
                <forename type="first">Thomas</forename>
                <surname>Charpentier</surname>
              </persName>
              <email type="md5">b1db3f89589e8150a11865470c953fac</email>
              <email type="domain">universite-paris-saclay.fr</email>
              <idno type="idhal" notation="string">thomas-charpentier</idno>
              <idno type="idhal" notation="numeric">1673339</idno>
              <idno type="halauthorid" notation="string">1748901-1673339</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-6373-6128</idno>
              <idno type="IDREF">https://www.idref.fr/269025693</idno>
              <affiliation ref="#struct-84494"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Séverine</forename>
                <surname>Boisard</surname>
              </persName>
              <idno type="halauthorid">730001-0</idno>
              <affiliation ref="#struct-84494"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Anne-Marie</forename>
                <surname>Le Ray</surname>
              </persName>
              <idno type="halauthorid">1076834-0</idno>
              <affiliation ref="#struct-84494"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Dimitri</forename>
                <surname>Bréard</surname>
              </persName>
              <email type="md5">00eb84a54348b7e37e47f9d099489946</email>
              <email type="domain">univ-angers.fr</email>
              <idno type="idhal" notation="numeric">784096</idno>
              <idno type="halauthorid" notation="string">1231216-784096</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-6477-9592</idno>
              <affiliation ref="#struct-84494"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Amélie</forename>
                <surname>Chabrier</surname>
              </persName>
              <email type="md5">1730d764d0efa9142dca3839588d4300</email>
              <email type="domain">unimes.fr</email>
              <idno type="idhal" notation="string">amelie-chabrier</idno>
              <idno type="idhal" notation="numeric">743312</idno>
              <idno type="halauthorid" notation="string">44539-743312</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Hélène</forename>
                <surname>Esselin</surname>
              </persName>
              <idno type="idhal" notation="numeric">1166609</idno>
              <idno type="halauthorid" notation="string">1581713-1166609</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-5691-0626</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">David</forename>
                <surname>Guilet</surname>
              </persName>
              <idno type="halauthorid">7105-0</idno>
              <affiliation ref="#struct-84494"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Christophe</forename>
                <surname>Ripoll</surname>
              </persName>
              <idno type="halauthorid">2311260-0</idno>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Pascal</forename>
                <surname>Richomme</surname>
              </persName>
              <email type="md5">7f0c866101be6fd46b3f1fc6de4e11ee</email>
              <email type="domain">univ-angers.fr</email>
              <idno type="idhal" notation="numeric">1167080</idno>
              <idno type="halauthorid" notation="string">191503-1167080</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-7788-2556</idno>
              <idno type="IDREF">https://www.idref.fr/081099703</idno>
              <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=s_44VHEAAAAJ</idno>
              <idno type="VIAF">https://viaf.org/viaf/194958545</idno>
              <idno type="ISNI">http://isni.org/isni/0000000139157580</idno>
              <affiliation ref="#struct-84494"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Anne-Marie</forename>
                <surname>Le Ray</surname>
              </persName>
              <email type="md5">15d0a4f6b6c76dcce0cf75ab033be384</email>
              <email type="domain">univ-angers.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2022-09-22 12:08:32</date>
              <date type="whenModified">2026-04-24 16:45:36</date>
              <date type="whenReleased">2022-09-22 14:44:58</date>
              <date type="whenProduced">2022-01</date>
              <date type="whenEndEmbargoed">2022-09-22</date>
              <ref type="file" target="https://univ-angers.hal.science/hal-03783689v1/document">
                <date notBefore="2022-09-22"/>
              </ref>
              <ref type="file" subtype="greenPublisher" n="1" target="https://univ-angers.hal.science/hal-03783689v1/file/A%20Descriptive%20Chemical%20Composition%20of%20Concentrated%20Bud%20Macerates%20through%20an%20Optimized%20SPE-HPLC-UV-MS2%20Method%20%28P%20-%202022%29.pdf" id="file-3783689-3310450">
                <date notBefore="2022-09-22"/>
              </ref>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="629896">
                <persName>
                  <forename>Anne-Marie</forename>
                  <surname>Le Ray</surname>
                </persName>
                <email type="md5">15d0a4f6b6c76dcce0cf75ab033be384</email>
                <email type="domain">univ-angers.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-03783689</idno>
            <idno type="halUri">https://univ-angers.hal.science/hal-03783689</idno>
            <idno type="halBibtex">charpentier:hal-03783689</idno>
            <idno type="halRefHtml">&lt;i&gt;Plants&lt;/i&gt;, 2022, 11 (2), pp.144. &lt;a target="_blank" href="https://dx.doi.org/10.3390/plants11020144"&gt;&amp;#x27E8;10.3390/plants11020144&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Plants, 2022, 11 (2), pp.144. &amp;#x27E8;10.3390/plants11020144&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://creativecommons.org/licenses/by/4.0/">CC BY 4.0 - Attribution<ref corresp="#file-3783689-3310450"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="UNIV-ANGERS">Université d'Angers</idno>
            <idno type="stamp" n="SONAS" corresp="UNIV-ANGERS">Substances d'Origine Naturelle et Analogues Structuraux</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">A Descriptive Chemical Composition of Concentrated Bud Macerates through an Optimized SPE-HPLC-UV-MS2 Method—Application to Alnus glutinosa, Ribes nigrum, Rosa canina, Rosmarinus officinalis and Tilia tomentosa</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Thomas</forename>
                    <surname>Charpentier</surname>
                  </persName>
                  <email type="md5">b1db3f89589e8150a11865470c953fac</email>
                  <email type="domain">universite-paris-saclay.fr</email>
                  <idno type="idhal" notation="string">thomas-charpentier</idno>
                  <idno type="idhal" notation="numeric">1673339</idno>
                  <idno type="halauthorid" notation="string">1748901-1673339</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-6373-6128</idno>
                  <idno type="IDREF">https://www.idref.fr/269025693</idno>
                  <affiliation ref="#struct-84494"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Séverine</forename>
                    <surname>Boisard</surname>
                  </persName>
                  <idno type="halauthorid">730001-0</idno>
                  <affiliation ref="#struct-84494"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Anne-Marie</forename>
                    <surname>Le Ray</surname>
                  </persName>
                  <idno type="halauthorid">1076834-0</idno>
                  <affiliation ref="#struct-84494"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Dimitri</forename>
                    <surname>Bréard</surname>
                  </persName>
                  <email type="md5">00eb84a54348b7e37e47f9d099489946</email>
                  <email type="domain">univ-angers.fr</email>
                  <idno type="idhal" notation="numeric">784096</idno>
                  <idno type="halauthorid" notation="string">1231216-784096</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-6477-9592</idno>
                  <affiliation ref="#struct-84494"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Amélie</forename>
                    <surname>Chabrier</surname>
                  </persName>
                  <email type="md5">1730d764d0efa9142dca3839588d4300</email>
                  <email type="domain">unimes.fr</email>
                  <idno type="idhal" notation="string">amelie-chabrier</idno>
                  <idno type="idhal" notation="numeric">743312</idno>
                  <idno type="halauthorid" notation="string">44539-743312</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Hélène</forename>
                    <surname>Esselin</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">1166609</idno>
                  <idno type="halauthorid" notation="string">1581713-1166609</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-5691-0626</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">David</forename>
                    <surname>Guilet</surname>
                  </persName>
                  <idno type="halauthorid">7105-0</idno>
                  <affiliation ref="#struct-84494"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Christophe</forename>
                    <surname>Ripoll</surname>
                  </persName>
                  <idno type="halauthorid">2311260-0</idno>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Pascal</forename>
                    <surname>Richomme</surname>
                  </persName>
                  <email type="md5">7f0c866101be6fd46b3f1fc6de4e11ee</email>
                  <email type="domain">univ-angers.fr</email>
                  <idno type="idhal" notation="numeric">1167080</idno>
                  <idno type="halauthorid" notation="string">191503-1167080</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-7788-2556</idno>
                  <idno type="IDREF">https://www.idref.fr/081099703</idno>
                  <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=s_44VHEAAAAJ</idno>
                  <idno type="VIAF">https://viaf.org/viaf/194958545</idno>
                  <idno type="ISNI">http://isni.org/isni/0000000139157580</idno>
                  <affiliation ref="#struct-84494"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">127755</idno>
                <idno type="issn">2223-7747</idno>
                <title level="j">Plants</title>
                <imprint>
                  <publisher>MDPI</publisher>
                  <biblScope unit="volume">11</biblScope>
                  <biblScope unit="issue">2</biblScope>
                  <biblScope unit="pp">144</biblScope>
                  <date type="datePub">2022-01</date>
                </imprint>
              </monogr>
              <idno type="doi">10.3390/plants11020144</idno>
              <ref type="publisher">https://www.mdpi.com/2223-7747/11/2/144</ref>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">Chabrier</term>
                <term xml:lang="en">Charpentier</term>
                <term xml:lang="en">T.</term>
                <term xml:lang="en">Boisard</term>
                <term xml:lang="en">S.</term>
                <term xml:lang="en">Le Ray</term>
                <term xml:lang="en">A.-M.</term>
                <term xml:lang="en">Bréard</term>
                <term xml:lang="en">D.</term>
                <term xml:lang="en">A.</term>
                <term xml:lang="en">antioxidant activity</term>
                <term xml:lang="en">flavonoid content</term>
                <term xml:lang="en">HPLC-UV-MS</term>
                <term xml:lang="en">P. A Descriptive concentrated bud macerates</term>
                <term xml:lang="en">Richomme</term>
                <term xml:lang="en">C.</term>
                <term xml:lang="en">Ripoll</term>
                <term xml:lang="en">Guilet</term>
                <term xml:lang="en">H.</term>
                <term xml:lang="en">Esselin</term>
              </keywords>
              <classCode scheme="halDomain" n="chim">Chemical Sciences</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Concentrated bud macerates (CBMs) are obtained from meristematic tissues such as buds and young shoots by maceration in a solvent composed of glycerin, water and ethanol (1/1/1/, v/v). Their traditional utilization in gemmotherapy has gained interest in the past years, and the knowledge of their chemical characterization can provide commercial arguments, particularly to secure their quality control. Therefore, an optimized method for phytochemical analysis including glycerol removal by a preliminary solid phase extraction (SPE) followed by compound identification using high performance liquid chromatography coupled with ultra-violet and tandem mass detectors (HPLC-UV-MS2) was developed. This method was applied on 5 CBMs obtained from Alnus glutinosa, Ribesnigrum, Rosmarinus officinalis, Rosa canina and Tilia tomentosa in order to determinate their chemical composition. Their antioxidant effects were also investigated by radical scavenging activity assays (DPPH and ORAC). Glycerol removal improved the resolution of HPLC chemical profiles and allowed us to perform TLC antioxidant screening. Our approach permitted the identification of 57 compounds distributed in eight major classes, three of them being common to all macerates including nucleosides, phenolic acids and glycosylated flavonoids. Quantification of the later class as a rutin equivalent (RE) showed a great disparity between Rosa canina macerate (809 mg RE/L), and the other ones (from 175 to 470 mg RE/L). DPPH and ORAC assays confirmed the great activity of Rosa canina (4857 and 6479 μmol TE/g of dry matter, respectively). Finally, phytochemical and antioxidant analysis of CBMs strengthened their phytomedicinal interest in the gemmotherapy field.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-84494" status="VALID">
          <idno type="IdRef">108015955</idno>
          <idno type="RNSR">199213267K</idno>
          <orgName>Substances d'Origine Naturelle et Analogues Structuraux</orgName>
          <orgName type="acronym">SONAS</orgName>
          <date type="start">1992-01-01</date>
          <desc>
            <address>
              <addrLine>Université d'Angers, SONAS - Campus du Végétal, 42 Rue Georges Morel - 49070 Beaucouzé</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://sonas.univ-angers.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-74911" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-74911" status="VALID">
          <idno type="IdRef">026402920</idno>
          <idno type="ISNI">0000000122483363</idno>
          <idno type="ROR">https://ror.org/04yrqp957</idno>
          <orgName>Université d'Angers</orgName>
          <orgName type="acronym">UA</orgName>
          <date type="start">1971-10-25</date>
          <desc>
            <address>
              <addrLine>Université d'Angers - 40 Rue de Rennes, BP 73532 - 49035 Angers CEDEX 01</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-angers.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>