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Article Dans Une Revue New Phytologist Année : 2012

Phytosphingosine-phosphate is a signal for AtMPK6 activation and Arabidopsis response to chilling

Christelle Dutilleul
  • Fonction : Auteur
Ghouziel Benhassaine-Kesri
  • Fonction : Auteur
Chantal Demandre
  • Fonction : Auteur
Nathalie Rézé
  • Fonction : Auteur
Alban Launay
  • Fonction : Auteur
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  • IdRef : 18006794X
Sandra Pelletier
Alain Zachowski
  • Fonction : Auteur
Emmanuel Baudouin
  • Fonction : Auteur
Isabelle Guillas

Résumé

Long-chain bases (LCBs) are pleiotropic sphingolipidic signals in eukaryotes. We investigated the source and function of phytosphingosine-1-phosphate (PHS-P), a phospho-LCB rapidly and transiently formed in Arabidopsis thaliana on chilling.PHS-P was analysed by thin-layer chromatography following in vivo metabolic radiolabelling. Pharmacological and genetic approaches were used to identify the sphingosine kinase isoforms involved in cold-responsive PHS-P synthesis. Gene expression, mitogen-activated protein kinase activation and growth phenotypes of three LCB kinase mutants (lcbk1, sphk1 and lcbk2) were studied following cold exposure. Chilling provoked the rapid and transient formation of PHS-P in Arabidopsis cultured cells and plantlets. Cold-evoked PHS-P synthesis was reduced by LCB kinase inhibitors and abolished in the LCB kinase lcbk2 mutant, but not in lcbk1 and sphk1 mutants. lcbk2 presented a constitutive AtMPK6 activation at 22°C. AtMPK6 activation was also triggered by PHS-P treatment independently of PHS/PHS-P balance. lcbk2 mutants grew comparably with wild-type plants at 22 and 4°C, but exhibited a higher root growth at 12°C, correlated with an altered expression of the cold-responsive DELLA gene RGL3. Together, our data indicate a function for LCBK2 in planta. Furthermore, they connect PHS-P formation with plant response to cold, expanding the field of LCB signalling in plants..

Dates et versions

hal-03339366 , version 1 (09-09-2021)

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Christelle Dutilleul, Ghouziel Benhassaine-Kesri, Chantal Demandre, Nathalie Rézé, Alban Launay, et al.. Phytosphingosine-phosphate is a signal for AtMPK6 activation and Arabidopsis response to chilling. New Phytologist, 2012, 194 (1), pp.181 - 191. ⟨10.1111/j.1469-8137.2011.04017.x⟩. ⟨hal-03339366⟩
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