%0 Journal Article %T Nitric Oxide and Calcium Participate in the Fine Regulation of Mitochondrial Biogenesis in Follicular Thyroid Carcinoma Cells %+ Mitochondrie : Régulations et Pathologie %+ Centre Hospitalier Universitaire d'Angers (CHU Angers) %+ Stress Oxydant et Pathologies Métaboliques (SOPAM) %+ Université d'Angers (UA) %+ Cardiopathies et mort subite [ERL 3147] %A Le Pennec, Soazig %A Mirebeau-Prunier, Delphine %A Bouzamondo, Nathalie %A Jacques, Caroline %A Guillotin, Delphine %A Lauret, Emilie %A Houlgatte, Rémi %A Malthièry, Yves %A Savagner, Frédérique %< avec comité de lecture %@ 0021-9258 %J Journal of Biological Chemistry %I American Society for Biochemistry and Molecular Biology %V 286 %N 20 %P 18229 - 18239 %8 2011 %D 2011 %R 10.1074/jbc.M110.217521 %K Calcium %K Gene %K mitochondrial %K Nitric %K thyroid %Z Life Sciences [q-bio]Journal articles %X
Members of the peroxisome proliferator-activated receptor γ coactivator-1 family (i.e. PGC-1α, PGC-1β, and the PGC-1-related coactivator (PRC)) are key regulators of mitochondrial biogenesis and function. These regulators serve as mediators between environmental or endogenous signals and the transcriptional machinery governing mitochondrial biogenesis. The FTC-133 and RO82 W-1 follicular thyroid carcinoma cell lines, which present significantly different numbers of mitochondria, metabolic mechanisms, and expression levels of PRC and PGC-1α, may employ retrograde signaling in response to respiratory dysfunction. Nitric oxide (NO) and calcium have been hypothesized to participate in this activity. We investigated the effects of the S-nitroso-N-acetyl-dl-penicillamine-NO donor, on the expression of genes involved in mitochondrial biogenesis and cellular metabolic functions in FTC-133 and RO82 W-1 cells by measuring lactate dehydrogenase and cytochrome c oxidase (COX) activities. We studied the action of ionomycin and 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid-acetoxymethyl ester (BAPTA/AM) (i.e. a calcium ionophore and a cytosolic calcium chelator) on whole genome expression and mitochondrial biogenesis in RO82 W-1 cells. COX activity and the dynamics of endoplasmic reticulum and mitochondrial networks were analyzed in regard to calcium-modulating treatments. In the FTC-133 and RO82 W-1 cells, the mitochondrial biogenesis induced by NO was mainly related to PRC expression as a retrograde mitochondrial signaling. Ionomycin diminished COX activity and negatively regulated PRC-mediated mitochondrial biogenesis in RO82 W-1 cells, whereas BAPTA/AM produced the opposite effects with a reorganization of the mitochondrial network. This is the first demonstration that NO and calcium regulate mitochondrial biogenesis through the PRC pathway in thyroid cell lines.
%G English %L hal-03275686 %U https://univ-angers.hal.science/hal-03275686 %~ UNIV-NANTES %~ CNRS %~ UNIV-ANGERS %~ UNAM %~ NANTES-UNIVERSITE %~ UNIV-NANTES-AV2022