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            <idno type="halRefHtml">&lt;i&gt;Pathologie-biologie&lt;/i&gt;, 2011, 59 (2), pp.e21-e27. &lt;a target="_blank" href="https://dx.doi.org/10.1016/j.patbio.2010.07.014"&gt;&amp;#x27E8;10.1016/j.patbio.2010.07.014&amp;#x27E9;&lt;/a&gt;</idno>
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                <title xml:lang="fr">Résultats de trois méthodes pour la détection de la mutation précore G1896A du virus de l’hépatite B chez les donneurs de sang français : PCR temps réel, séquençage et test Inno-LIPA</title>
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                  <persName>
                    <forename type="first">Alexandra</forename>
                    <surname>Ducancelle</surname>
                  </persName>
                  <email type="md5">58badbc19a669aaa3f386ac6867f7f8d</email>
                  <email type="domain">univ-angers.fr</email>
                  <idno type="idhal" notation="numeric">992141</idno>
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                    <forename type="first">A.</forename>
                    <surname>Servant-Delmas</surname>
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                  <idno type="halauthorid">439989-0</idno>
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                  <persName>
                    <forename type="first">T.</forename>
                    <surname>Beuvelet</surname>
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                  <idno type="halauthorid">2235716-0</idno>
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                  <persName>
                    <forename type="first">Viorica</forename>
                    <surname>Balan</surname>
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                  <idno type="halauthorid">1075726-0</idno>
                  <affiliation ref="#struct-245050"/>
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                  <persName>
                    <forename type="first">Adeline</forename>
                    <surname>Pivert</surname>
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                  <email type="md5">ece1eaa6e94fae23a57b44174df76f99</email>
                  <email type="domain">chu-angers.fr</email>
                  <idno type="idhal" notation="numeric">992791</idno>
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                    <forename type="first">Michèle</forename>
                    <surname>Maniez</surname>
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                  <idno type="halauthorid">2235235-0</idno>
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                <author role="aut">
                  <persName>
                    <forename type="first">Syria</forename>
                    <surname>Laperche</surname>
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                  <idno type="halauthorid">318089-0</idno>
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                  <persName>
                    <forename type="first">Françoise</forename>
                    <surname>Lunel-Fabiani</surname>
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                  <email type="md5">63b24133997514a51d3bca2dddff2130</email>
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                  <idno type="idhal" notation="numeric">992126</idno>
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                <title level="j">Pathologie-biologie</title>
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                  <date type="datePub">2011</date>
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                <term xml:lang="fr">Diagnostic</term>
                <term xml:lang="fr">Adolescent</term>
                <term xml:lang="fr">Adult</term>
                <term xml:lang="fr">Africa</term>
                <term xml:lang="fr">Asia</term>
                <term xml:lang="fr">Blood Donors</term>
                <term xml:lang="fr">Computer Systems</term>
                <term xml:lang="fr">DNA Mutational Analysis</term>
                <term xml:lang="fr">Young Adult</term>
                <term xml:lang="fr">Viremia</term>
                <term xml:lang="fr">DNA</term>
                <term xml:lang="fr">Sequence Analysis</term>
                <term xml:lang="fr">Europe</term>
                <term xml:lang="fr">Reagent Kits</term>
                <term xml:lang="fr">Polymerase Chain Reaction</term>
                <term xml:lang="fr">Point Mutation</term>
                <term xml:lang="fr">Middle Aged</term>
                <term xml:lang="fr">Mass Screening</term>
                <term xml:lang="fr">Male</term>
                <term xml:lang="fr">Immunoenzyme Techniques</term>
                <term xml:lang="fr">Humans</term>
                <term xml:lang="fr">hepatitis B virus</term>
                <term xml:lang="fr">Hepatitis B Surface Antigens</term>
                <term xml:lang="fr">Hepatitis B</term>
                <term xml:lang="fr">Genotype</term>
                <term xml:lang="fr">France</term>
                <term xml:lang="fr">Female</term>
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              <p>&lt;p&gt;&lt;strong&gt;AIM: &lt;/strong&gt;To screen hepatitis B virus (HBV) genotypes and associated basal core promoter (BCP; T1762A/A1764) and precore (PC; A1896) mutations among the 100 HBV surface antigen (HBsAg) positive voluntary blood donors in France.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;METHODS: &lt;/strong&gt;HBV genotypes were determined by using direct sequence analysis. Three methods were used to detect G1896A mutation: non-commercial real-time PCR (PCRTR°, line probe assay (InnoLiPA HBV PreCore, INNOGENETICS(®)) and direct sequencing of precore gene. HBV viral load was quantified with two commercial real-time PCR (COBAS(®) AmpliPrep/COBAS(®) TaqMan(®) HBV Test/Roche and Real Time HBV/M2000/Abbott).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;RESULTS: &lt;/strong&gt;The mean age of donors was 30 (18-64). Patients were from Africa (42%), Europa (50%), and Asia (8%). HBV/D was the most predominant (37%) genotype followed by HBV/A (31%) and HBV/E (22%). PC and BCP mutants were found in 57% with Inno-LIPA HBV test and 59% with both PCRTR and sequencing methods. A significant difference in the viral load of blood donors with wild and PC mutants was observed with the Taqman Cobas real time PCR (3,19 Log(10) UI/ml versus 4,93 Log(10) UI/ml, p&amp;nbsp;&amp;lt;&amp;nbsp;0.05). Precore phenotype determination was in agreement with the three PC mutation detection methods in 56% of cases.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;CONCLUSIONS: &lt;/strong&gt;Non-Caucasian genotype E was present in the French blood donors. PC mutation was more common than BCP mutations in this study. As HBV infected blood donors were more often asymptomatic carriers, we could speculate that the G1896A mutation may favour the asymptomatic state, supporting previous observations.&lt;/p&gt;</p>
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              <p>&lt;p&gt;&lt;strong&gt;Buts du travail&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;Plusieurs études internationales ont décrit une prévalence élevée de la mutation précore G1896A (PC) chez les donneurs de sang. Nous avons mené un projet en collaboration avec l’Institut national de transfusion sanguine sur la prévalence des génotypes et des mutants précore chez les donneurs de sang en France.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Patients et méthodes&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;L’effectif comprenait 100&amp;nbsp;donneurs de sang nouvellement dépistés AgHBs positifs. La mutation PC a été recherchée par trois méthodes différentes&amp;nbsp;: une technique de PCR temps réel (PCR TR) développée dans notre laboratoire et ciblée sur la mutation PC, le séquençage direct et l’hybridation moléculaire inverse (Kit Inno-LIPA HBV Precore, INNOGENETICS®).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Résultats&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;La moyenne d’âge dans la population étudiée était de 30&amp;nbsp;ans (extrêmes 18–64). L’origine géographique était africaine dans 42&amp;nbsp;% des cas. La prévalence de la mutation G1896A isolée ou associée à du virus précore sauvage était de 57&amp;nbsp;% en technique Inno-LIPA et de 59&amp;nbsp;% par séquençage ou PCR TR. Les donneurs infectés par un virus précore muté avaient des charges virales VHB significativement plus basses que les patients porteurs d’un virus sauvage (3,19&amp;nbsp;Log10&amp;nbsp;UI/ml versus 4,93&amp;nbsp;Log10&amp;nbsp;UI/ml, p&amp;nbsp;&amp;lt;&amp;nbsp;0,05).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;Nous confirmons les données de la littérature à savoir une prévalence relativement élevée de mutation PC chez les donneurs de sang français. Sachant que ces sujets sont la plupart du temps asymptomatiques et avec des charges virales VHB inférieures au seuil défini par l’EASL pour le portage inactif, la question du rôle de la mutation G1896A dans la sévérité des lésions hépatiques reste à préciser.&lt;/p&gt;</p>
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