Aluminum inhibits the growth of hydroxyapatite crystals developed on a biomimetic methacrylic polymer. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Trace Elements in Medicine and Biology Année : 2013

Aluminum inhibits the growth of hydroxyapatite crystals developed on a biomimetic methacrylic polymer.

, (1) , (1) , (1)
1

Résumé

PROJECT: Aluminum (Al) is an increasing problem in biomedicine since it can interact with phosphates. Bone is one of the preferential target tissues of Al deposition: Al interacts with mineralization and/or bone cell activities. We searched the influence of Al deposition in hydroxyapatite developed on a biomimetic polymer (carboxymethylated poly(2-hydroxyethyl-methacrylate)) which mimics bone mineralization in the absence of cells.

PROCEDURES: Pellets of polymer were incubated for 5 days in a synthetic body fluid (SBF) to induce mineralization, then 21 days in SBF containing 20, 40 and 60 μg/L Al(3+). Other pellets were incubated in SBF containing commercial Al foil (33 mg/vial) either in 1, 2 or 6 pieces. The mineral deposits were dissolved in HCl and Ca(2+), PO(4)(3-) and Al(3+) content was measured. Hydroxyapatite was characterized by SEM and X energy-dispersive X-ray analysis (EDX).

RESULTS: The amount of Al(3+) was dose-dependently increased in Ca/P deposits on the polymer pellets. At high concentration (or with the 6 Al foils) growth of hydroxyapatite calcospherite was inhibited; only calcified plates emerging from the polymer were observed. Pellets incubated with 1 and 2 Al foils exhibited a reduction in calcospherite diameter and an increase in the Al(3+)/Ca(2+) ratio. EDX identified Al in the mineral deposits.

CONCLUSIONS: In this acellular model, Al(3+) altered the growth of calcospherites at low concentration and inhibited their development at high concentration. In SBF, a release of Al(3+) from aluminum foils also inhibited mineralization. This study emphasizes the importance of Al in bone pathology and stresses the question of its release from biomaterials.

Fichier principal
Vignette du fichier
2013-jtraceelementsmedbiol.pdf (952.08 Ko) Télécharger le fichier
Origine : Accord explicite pour ce dépôt

Dates et versions

hal-03265946 , version 1 (21-06-2021)

Identifiants

Citer

Cristinel Nicolae Degeratu, Guillaume Mabilleau, Corneliu Cincu, Daniel Chappard. Aluminum inhibits the growth of hydroxyapatite crystals developed on a biomimetic methacrylic polymer.. Journal of Trace Elements in Medicine and Biology, 2013, 27, pp.346-51. ⟨10.1016/j.jtemb.2013.05.004⟩. ⟨hal-03265946⟩

Collections

UNIV-ANGERS
7 Consultations
76 Téléchargements

Altmetric

Partager

Gmail Facebook Twitter LinkedIn More